Identification and characterisation of lamprey protein kinase C delta-like gene

Yang Xu,Qingwei Li,Huan Zhao,Siwei Zhu

doi:10.1038/s41598-017-12526-w

Abstract

Protein kinase C-δ (PKC-δ), a member of the lipid-regulated serine/threonine PKC family, has been implicated in a wide range of important cellular processes, such as cell growth, differentiation, and apoptosis. Lampreys belong to the most primitive class of vertebrates, and there is little information on PKC-δ in these animals. In this study, a PKC-δ-like cDNA sequence and deduced PKC-δ-like amino acid sequence were identified in the Japanese lamprey (Lampetra japonica). The PKC-δ-like gene shared approximately 60% sequence identity with its homologs in jawed vertebrates. The anti-PKC-δ-like polyclonal antibodies were well prepared, and experiments showed that PKC-δ-like was primarily distributed in the supraneural body of the lamprey. Both mRNA and protein levels of PKC-δ-like in supraneural body cells were increased after incubation with cis-diaminedichloroplatinum (CDDP). Moreover, PKC-δ-like protein induced the apoptosis of HEK-293T cells. In addition, the activation of PKC-δ-like resulted in apoptosis. Conversely, the inhibition of PKC-δ-like activity disrupted the CDDP-mediated induction of cellular apoptosis. These results indicate that PKC-δ-like identified in lampreys might play an important role in apoptosis in jawless vertebrates.

Highlights

Protein kinase C (PKC), a family of phospholipid-dependent serine/threonine protein kinases, comprises a multigene family of related serine/threonine kinases that sit at the crossroads of many signal transduction pathways and regulate a wide variety of cellular functions, including cell proliferation, differentiation and cell death
Sequence homologous to human Protein kinase C-δ (PKC-δ) was identified among the extensive expressed sequence tags (EST) sequences obtained from the cDNA library of L. japonica supraneural body
We performed a BLASTp analysis of the full-length protein sequence of PKC-δ-like, and all of the significant alignments were with PKC-δ homologs of different species with high identities

Summary

Introduction

Protein kinase C (PKC), a family of phospholipid-dependent serine/threonine protein kinases, comprises a multigene family of related serine/threonine kinases that sit at the crossroads of many signal transduction pathways and regulate a wide variety of cellular functions, including cell proliferation, differentiation and cell death. Studies have shown that the supraneural body is associated with haematopoiesis but is involved in lamprey immunity because numerous immune molecules have been identified in this organ[25,26,27] Because of their unique position at the interface between jawless and jawed vertebrates, lampreys are considered one of the most important research models for understanding the origin and evolution of vertebrates[28,29]. The potential role of PKC-δ-like in the apoptosis of lamprey cells was studied

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Conclusion