Identification and characterisation of chicken cDNAs encoding the endothelial cell-specific receptor tyrosine kinase Tie2 and its ligands, the angiopoietins.

Abstract

The angiopoietins represent a recently discovered family of angiogenic factors that recognise and specifically bind to the endothelial cell-specific Tie2 receptor tyrosine kinase. The specific biological functions of the angiopoietins have not yet been elucidated, although transgenic technology has shown the critical roles that these proteins have in vascularisation. The chicken vasculature, and more particularly the chorioallantoic membrane, is an important tool in the study of angiogenesis and has been previously used to study the functions of angiogenic factors such as vascular endothelial growth factor. We have undertaken the identification of the chicken orthologs of the angiopoietins and the Tie2 receptor. cDNA clones that encompass the full coding sequence of chicken Tie2 show features typical of this family of receptor tyrosine kinases. Chicken angiopoietin-1 and angiopoietin-2 show a high degree of homology to their human counterparts, 91% and 87% respectively, a reflection of the critical role of this signalling pathway.