Abstract
Chemical epigenetic manipulation was applied to a deep marine-derived fungus, Aspergillus sp. SCSIOW3, resulting in significant changes of the secondary metabolites. One new diphenylether-O-glycoside (diorcinol 3-O-α-D-ribofuranoside), along with seven known compounds, were isolated from the culture treated with a combination of histone deacetylase inhibitor (suberohydroxamic acid) and DNA methyltransferase inhibitor (5-azacytidine). Compounds 2 and 4 exhibited significant biomembrane protective effect of erythrocytes. 2 also showed algicidal activity against Chattonella marina, a bloom forming alga responsible for large scale fish deaths.
Highlights
With the recent completion of fungal genomes, it has become clear that the number of gene clusters encoding secondary metabolites greatly outnumbers the characterized compounds from these organisms [1]
This is the first report about diphenyl esters against Chattonella marina
The present study aimed at activating silent biosynthetic pathways of secondary metabolites and identifying active novel compounds from fungi
Summary
With the recent completion of fungal genomes, it has become clear that the number of gene clusters encoding secondary metabolites greatly outnumbers the characterized compounds from these organisms [1]. We successfully obtained four new eremophilane-type we successfully from obtained fourmarine new eremophilane-type sesquiterpenes from a deep marine derived sesquiterpenes a deep derived fungi, Aspergillus sp. Combination of SBHA and 5-AZA [8]. One member of this set, Aspergillus sp. EtOAc extract of this culture was scaled-up and separated for further study. The EtOAc extract of this culture was scaled-up and separated by by using column chromatography and semi-preparativeHPLC, HPLC,resulting resultingininthe theisolation isolation of of one one new using column chromatography and semi-preparative new compound, diorcinol. EtOAc extracts of untreated (a) and 5-Aza. EtOAc extracts of untreated (a) and 5-Aza. (A) HPLC-UV chromatogram at 254 nm of EtOAc extracts of untreated (a) and SBHA 1+
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