Identification and Bioinformatic Analysis of the GmDOG1-Like Family in Soybean and Investigation of Their Expression in Response to Gibberellic Acid and Abscisic Acid.

Yingzeng Yang,Tian Pu,Chunyan Liu,Umashankar Chandrasekaran,Kai Shu,Liang Yu,Weiguo Liu,Wenyu Yang,Xiaochun Wang,Taiwen Yong,Junbo Du,Feng Yang,Chuan Zheng,Jiang Liu

doi:10.3390/plants9080937

Yingzeng Yang, Tian Pu + Show 12 more

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https://doi.org/10.3390/plants9080937

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Abstract

Seed germination is one of the most important stages during plant life cycle, and DOG1 (Delay of germination1) plays a pivotal regulatory role in seed dormancy and germination. In this study, we have identified the DOG1-Like (DOG1L) family in soybean (Glycine max), a staple oil crop worldwide, and investigated their chromosomal distribution, structure and expression patterns. The results showed that the GmDOG1L family is composed of 40 members, which can be divided into six subgroups, according to their evolutionary relationship with other known DOG1-Like genes. These GmDOG1Ls are distributed on 18 of 20 chromosomes in the soybean genome and the number of exons for all the 40 GmDOG1Ls varied greatly. Members of the different subgroups possess a similar motif structure composition. qRT-PCR assay showed that the expression patterns of different GmDOG1Ls were significantly altered in various tissues, and some GmDOG1Ls expressed primarily in soybean seeds. Gibberellic acid (GA) remarkably inhibited the expression of most of GmDOG1Ls, whereas Abscisic acid (ABA) inhibited some of the GmDOG1Ls expression while promoting others. It is speculated that some GmDOG1Ls regulate seed dormancy and germination by directly or indirectly relating to ABA and GA pathways, with complex interaction networks. This study provides an important theoretical basis for further investigation about the regulatory roles of GmDOG1L family on soybean seed germination.

Highlights

Soybean is an important legume plant, and is widely grown in many countries as a staple oil crop [1]
By using the NCBI CDD website, we found that the 40 GmDOG1L genes contained the conservative structural domain of DOG1(PF14144), which suggest that our analysis is reliable
We found that GmDOG1-L4, GmDOG1-L19, GmDOG1-L25, GmDOG1-L27, GmDOG1-L32 and GmDOG1-L40 were highly expressed in all tissues, implying that these genes might be important throughout the soybean life cycle

Summary

Introduction

Soybean is an important legume plant, and is widely grown in many countries as a staple oil crop [1]. Seed germination is an important stage during the plant life cycle, which contributes to the spread and distribution of wild species and enhance the quality and yield of cultivated crops [3]. Most angiosperms begin a new stage of growth and development after seed dormancy, which is a very useful survival mechanism protecting plants from adverse environments [4,5]. Successful germination of seeds in the field is essential for the stable high yield [6]. The intensive and extensive investigation of the regulatory mechanisms of seed dormancy and germination has an important scientific significance

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