Abstract
Chondroitinases, catalyzing the degradation of chondroitin sulfate (CS) into oligosaccharides, not only play a crucial role in understanding the structure and function of CS, but also have been reported as a potential candidate drug for the treatment of high CS-related diseases. Here, a marine bacterium Vibrio hyugaensis LWW-1 was isolated, and its genome was sequenced and annotated. A chondroitinase, VhChlABC, was found to belong to the second subfamily of polysaccharide lyase (PL) family 8. VhChlABC was recombinant expressed and characterized. It could specifically degrade CS-A, CS-B, and CS-C, and reached the maximum activity at pH 7.0 and 40 °C in the presence of 0.25 M NaCl. VhChlABC showed high stability within 8 h under 37 °C and within 2 h under 40 °C. VhChlABC was stable in a wide range of pH (5.0~10.6) at 4 °C. Unlike most chondroitinases, VhChlABC showed high surfactant tolerance, which might provide a good tool for removing extracellular CS proteoglycans (CSPGs) of lung cancer under the stress of pulmonary surfactant. VhChlABC completely degraded CS to disaccharide by the exolytic mode. This research expanded the research and application system of chondroitinases.
Highlights
Chondroitin sulfate (CS), a kind of glycosaminoglycan that can covalently link with protein to form proteoglycan, is widely distributed in the extracellular matrix and cell surface of animal tissues [1,2]
The CS chain is made up of disaccharide units consisting of D-glucuronic acid (GlcUA)/L-iduronic acid (IdoUA) linked to N-acetyl-D-galactosamine (GalNAc) and sulfated at the C2 position of uronic acid and/or at the C4/C6 position of GalNAc residue [8]
Strain LWW-1 showed the highest similarity of 99.64% with the Vibrio hyugaensis strain 090810a (Accession Number: LC004912)
Summary
Chondroitin sulfate (CS), a kind of glycosaminoglycan that can covalently link with protein to form proteoglycan, is widely distributed in the extracellular matrix and cell surface of animal tissues [1,2]. CS plays an important regulatory role in many physiological processes such as cell development, cell adhesion, proliferation and differentiations [3,4,5,6,7]. Important applications of CS in biological tissue engineering have been explored, which involve combining it with other biopolymers to form scaffolds capable of facilitating and accelerating the regeneration of damaged structures [3]. The structure of the main chain of polysaccharides is not complex, it shows a high degree of heterogeneity in terms of the degree of sulfation, the sulfation position, and the distribution of the two kinds of different isoguronic acids within the chain. Existing studies have indicated the interaction effect between CS and various growth factors/adhesion
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
