“Identification and analysis of major street fast food borne pathogenic fungi and its sensitivity of Nystatin”

Abstract

Street foods consuming is available in Bangladesh, mainly in tea stalls and among the fast foods bread, pates, salty biscuit, cake, sweet biscuit, chatpati and pickle are popular. As these popular foods are available at street side, these are prone to have microbial attack. We approached to determine the presence of pathogens responsible for food spoilage by inoculating samplesinto Potato-Dextrose-Agar (PDA) media in Laminar Air Flow facility at Bangladesh Agricultural Research Institute's Laboratoty. We made three replications of fungi in every seven days of interval using the PDA media. In each replication we obtained several types of fungi. Later on evaluating the intensity of the microbes we undertook pure culture and got Aspergillus flavus, Aspergillus niger and Fusarium sp., generally which are considered as the most frequently growing fungi. A sophisticated camera attached light microscope used to detect the fungi for proper characterization. Then we tested anti-fungal sensitivity using an anti-fungal agent Nystat (Nystatin at 100, 000 units/ml). Aspergillus flavus, Aspergillus niger and Fusarium sp. were collected from pure culture medias and we found Aspergillus flavus and Fusarim spp. as resistance to the aforementioned anti-fungal agent while A. niger remained sensitive to that agent. Isolation of major typical toxins mainly the aflatoxins and analysis of their molecular modeling using bioinformatic tools might be an epoch making event for us in ensuring food safety for all and new drug designing in future.