Abstract
The alterations of MicroRNAs(miRNAs) in host cell after foot-and-mouth disease virus (FMDV) infection is still obscure. To increase our understanding of the pathogenesis of FMDV at the post-transcriptional regulation level, Solexa high-throu MicroRNAs (miRNAs) play an important role both in the post-transcriptional regulation of gene expression and host-virus interactions. Despite investigations of miRNA expression ghput sequencing and bioinformatic tools were used to identify differentially expressed miRNAs and analyze their functions during FMDV infection of PK-15cells. Results indicated that 9,165,674 and 9,230,378 clean reads were obtained, with 172 known and 72 novel miRNAs differently expressed in infected and uninfected groups respectively. Some of differently expressed miRNAs were validated using stem-loop real-time quantitative RT-PCR. The GO annotation and KEGG pathway analysis for target genes revealed that differently expressed miRNAs were involved in immune response and cell death pathways.
Highlights
MicroRNAs are small 21–24 nucleotide noncoding RNAs that play an important role in the post-transcriptional regulation of gene expression [1,2]
Highthroughput sequencing has become a powerful strategy for identifying novel miRNAs and studying the expression profiles of miRNA in different samples
MiRNA represents a vital component of the innate antiviral response in virus infection [31].It serves as a host gene-regulation mechanism triggered by the expression of structured miRNA molecules [11]
Summary
MicroRNAs (miRNAs) are small 21–24 nucleotide (nt) noncoding RNAs that play an important role in the post-transcriptional regulation of gene expression [1,2]. Mature miRNAs are not directly produced by gene transcription, they are processed from long primary miRNA (pri-miRNA) precursors. They are known to play a key role in biological activities at the molecular level [3,4,5]. The stability of miRNAs along with their ability to regulate gene expression is beneficial in developing breeding programs for disease resistance in livestock [8]
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