Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells.

Erwei Hao,Zhengcai Du,Yan Xie,Jianfeng Qin,Hangxuan Wu,Xiaosu Fan,Xiaotao Hou,Wei Wei,Jiagang Deng,Jinling Xie,Jianhua Miao,Xianglong Pan

doi:10.1155/2020/5165631

Abstract

Yizhi Granule (YZG) is a health food containing six traditional Chinese medicines (TCMs). It improves memory barriers in rat experiments. Here, we describe the first fast and sensitive ultraperformance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) method for analyzing YZG in plasma. We used this technique for studies in cynomolgus monkey plasma. By comparing retention time, MS, and MS/MS data of reference compounds, 70 compounds were detected in YZG. Of these, 63 were identified including 60 saponins, 2 flavones, and 1 methyl ester. There were 33 saponins, 1 flavone, and 1 methyl ester in the plasma. Next, to study the therapeutic properties of YZG, the neuroprotective effect of some of the absorbed components was evaluated using PC12 cell damage caused by the Aβ25–35 model. The results showed that 9 compounds protect PC12 cells from Aβ25–35 with cell viability (%) of 111.00 ± 8.12 (G-Rb1), 102.20 ± 4.22 (G-Rb2), 100.34 ± 6.47 (G-Rd), 102.83 ± 2.10 (G-Re), 101.68 ± 7.64 (NG-Fa), 101.19 ± 7.83 (NG-R1), 102.53 ± 0.55 (NG-R2), 106.88 ± 4.95 (gypenoside A), and 103.95 ± 4.11 (gypenoside XLIX), respectively, versus the control group (87.51 ± 6.59). These results can reveal the real pharmacodynamic basis of YZG and provide a theoretical basis for subsequent studies. It can also provide some references for the research of Alzheimer's disease.

Highlights

Alzheimer’s disease (AD) is a common chronic progressive neurodegenerative disease leading to memory impairment, hypophrenia, behavioral personality degeneration, disability, and premature death [1,2,3]. e prevalence of AD in China was 3.21% among people aged 65 years and older [4], and more than 7 million Chinese people live with AD today [5]
We recently showed that YZG improved memory barriers in animal experiments, and YZG could protect the PC12 from the damage induced by protein Aβ25-35 [12,13,14,15]; the active ingredients remain unclear [16]
We recently showed that YZG improved memory barriers in animal experiments and YZG could protect the PC12 from the damage induced by protein Aβ25–35 [12,13,14,15]. e related effects of these six herbs in YZG have been reported

Summary

Introduction

Alzheimer’s disease (AD) is a common chronic progressive neurodegenerative disease leading to memory impairment, hypophrenia, behavioral personality degeneration, disability, and premature death [1,2,3]. e prevalence of AD in China was 3.21% among people aged 65 years and older [4], and more than 7 million Chinese people live with AD today [5]. Alzheimer’s disease (AD) is a common chronic progressive neurodegenerative disease leading to memory impairment, hypophrenia, behavioral personality degeneration, disability, and premature death [1,2,3]. AD is a serious health problem for the elderly and a severe social problem. All AD patients undergo progressive β-amyloid deposition followed by surrounding neuritic and glial cytopathology in brain regions serving memory and cognition [6]. Traditional Chinese medicine (TCM) plays a vital role in treating Alzheimer’s disease in China.

Results

Discussion

Conclusion