Abstract
The objective of this study was to identify and quantify bacteria from fresh ram semen, evaluate the use of sanitizer Kilol-L® prior to semen collection, and test the sensitivity of bacterial strains against antibiotics. We selected 24 Santa Inês rams clinically healthy, at 4 years of aged, and grouped them into two systems: on pasture (n=12) and confined (n=12). The microbiological results indicated that of the total of 120 ejaculates, 99 had bacterial growth, representing 82.5% of the samples. The most frequently isolated bacteria were Bacillus spp., Corynebacterium spp., Escherichia coli, Listeria spp., Staphylococcus spp., and Streptococcus spp. From the tested antibiotics, amicacin and gentamicin were 100% effective for Bacillus spp. and E. Coli. Ceftiofur was effective for all isolates except for Rodococcus equi. Streptococcus spp. were susceptible to ampicillin and erythromycin and Staphylococcus spp. were sensitive to gentamycin. The use of Kilol-L® reduced the number of Colony Forming Units (CFU/mL) from ejaculate without damaging semen quality. From the tested antibiotics, ceftiofur and gentamicin were more effective against the isolated bacterial strains; thus the use of these antibiotics in the ram semen extender is an alternative to control bacterial growth.KEYWORDS: antibiogram; bacteria; ovine; sanitizer; semen sperm parameters.
Highlights
Colony Forming Units (CFU/mL) from ejaculate without isolated bacterial strains; the use of these antibiotics damaging semen quality
In the ram semen extender is an alternative to control ceftiofur and gentamicin were more effective against the bacterial growth
A uretra é parte comum do sistema urinário e genital, consequentemente a contaminação do sêmen, pode ter origem em ambos os sistemas
Summary
GABRIELA DE OLIVEIRA FERNANDES1, DIOGO RAMOS LEAL1, NATHALIA HACK MOREIRA1, THIAGO ANTONIO DE SOUZA NASCIMENTO SILVA1, ALEXANDRE FLORIANI RAMOS2, JAIRO PEREIRA NEVES3. O objetivo deste trabalho foi identificar e quantificar as bactérias do sêmen fresco de ovino, avaliar o uso do higienizante Kilol-L® antes da coleta do sêmen e testar a sensibilidade das cepas bacterianas frente ao antibiograma. Dos 120 ejaculados coletados, 99 tiveram crescimento bacteriano correspondendo a 82,5% das amostras. Os gêneros bacterianos isolados com maior frequência foram Staphylococcus spp., Bacillus spp., Streptococcus spp., Corynebacterium spp., Listeria spp.e Escherichia coli. A amicacina e a gentamicina foram 100% eficazes para Bacillus spp. e E. coli. O ceftiofur foi efetivo para todas as bactérias isoladas, exceto Rodococcus equi. Ceftiofur e gentamicina foram os mais efetivos frente às cepas bacterianas isoladas. Dessa forma, a utilização desses antibióticos no meio diluidor do sêmen ovino é uma alternativa para controlar o crescimento bacteriano. IDENTIFICATION OF BACTERIA IN THE SEMEN IN DIFFERENT BREEDING SYSTEMS AND THE EFFECT OF KILOL-L®
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