Abstract
With the objective of identifying the presence of Paratuberculosis (PTB), an infectious disease caused by Mycobacterium avium ssp paratuberculosis (MAP), in sheep, through pathological studies, bacterial culture and IS900 PCR, as well as estimating seroprevalence to MAP. The present cross-sectional study, was conducted in 16 different flocks, with the serum of 2415 adult sheeps, and analyzed by Enzyme-Linked ImmunoSorbent Assay (ELISA); nine sheep were used with clinical signs suggestive of PTB, from which samples were obtained for the identification studies; obtaining 51.3% of animals seropositive to MAP (1239/2415), in 100% of the herds (16/16); Bacterial isolation and its identification by PCR IS900 were founded in five of the nine cases (5/9) corresponding to 31.25% of the herds (5/16). Confirming the presence of Mycobacterium avium ssp paratuberculosis, and a high frequency of seropositive animals to MAP in flocks of Aguascalientes.
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