Identificación de Norovirus Humano (HNoV) en muestras de estiércol de cerdos domésticos.

Abstract

<p><strong><span style="font-family: Arial; font-size: small;">Objective. </span></strong><span style="font-family: Arial;">To determine the presence of NoVs as a possible causal zoonotic agent of acute diarrhea in pigs and humans. <strong>Materials and methods.</strong> We collected a total of 77 samples from diarrheal children under 5 years and pigs under 2 months from La Chamba town in Tolima, Colombia. These samples were transported to the Laboratory of Virology of the Pontificia Universidad Javeriana in Bogotá, and extraction with Trizol-reagent was done following the manufacturer's instructions. After obtaining the RNA, the next step was to perform RT-PCR for obtaining the expected amplification product of 213- bp NoVs. Finally, the positive samples obtained in the RT-PCR were sequenced and analyzed by bioinformatics methods. <strong>Results.</strong> Six positive diarrheic samples from children and a positive diarrheic sample from pigs were detected by a band of 231 bp. Five of the six positive samples in children and the positive pig sample were sequenced and analyzed. <strong>Conclusion.</strong> Given the close genetic relationship between pig and human sequences, this could be an indication of the potential existence of a common animal acting as a reservoir for human or other animal strains.</span></p> <p><span style="color: black; font-family: Arial; font-size: small;"> </span></p> <p><strong><span style="font-family: Arial; font-size: small;">Key words: </span></strong><span style="font-family: Arial;">Human Norovirus, domestic pigs, diarrhea, zoonotic transmission, reservoirs</span></p>