Ide (Leuciscus idus) sperm short-term storage: Effects of different extenders and dilution ratios

Abstract

In cyprinids, Tyrode’s (TLP) and Volckaert’s (VRT) solutions are the most frequently used extenders for short-term sperm storage. The effectiveness of TLP and VRT on ide (Leuciscus idus) sperm short-term storage was analyzed using a × 4 (sperm:extender) dilution ratio over 48 h. A × 4 (1:3) dilution ratio was compared to a × 10 (1:9) dilution ratio for ide sperm storage using TLP supplemented with antibiotics and was tested for a 14-day period. Sperm motility (MOT, %), progressively motile sperm (PRG, %), curvilinear velocity of sperm (VCL, µm s-1), movement linearity (LIN, %), beat cross frequency (BCF, Hz), and the amplitude of lateral head displacement (ALH, µm) were verified using a CASA system. After 48 h, most CASA parameters were significantly higher in TLP compared to VRT. The dilution ratio also had a significant impact (P < 0.0001) on the efficiency of ide sperm short-term storage over 14 d in comparison to undiluted sperm (control). Ide sperm diluted with TLP supplemented with penicillin/streptomycin and stored short term, regardless of the dilution ratio used, retained motility and fertilization capacity over 14 d at 4 °C. The highest embryo survival rates of 70% and 73% were noted using sperm diluted with TLP at × 4 and × 10 dilution ratios compared to 48% for raw sperm that was not stored short term.