IDDF2019-ABS-0030 Biosynthesis of silver nanoparticles using plant nepenthes spp. and its bactericidal effect

Abstract

Background The use of nanotechnology has grown rapidly for the past few years and has been implemented in various types of area including healthcare. The main aim of the proposed study is to synthesize safe and stable nanosilver by using the extract of plant Nepenthes spp. These nanoparticles will be characterized by various microscopic techniques to study the size and nature of nanoparticles. These nanoparticles will further be evaluated for its antibacterial activity against various bacterial pathogens and also evaluate its synergistic effect with ciprofloxacin. Methods 1. Collection of plant material. 2. Extraction 3. Biosynthesis of silver nanoparticles. 4. Characterization of silver nanoparticles. 4.1 UV-Vis Spectroscopy 4.2 Transmission Electron Microscope (TEM) Spectroscopy 5. Determination of anti-microbial activity of AgNPs by disc diffusion method. 5.1 Preparation of agar plate 5.2 Bacterial strain was cultured. 5.3 Antimicrobial activity test 5.4 Synergistic effect test Results Colour change observed in the leaves sample of Nepenthes spp. at different time intervals upon addition of AgNO3.UV-Vis spectrophotometer analysis was used to detect and confirmed the presence of silver nanoparticles and the reduction of silver ion into silver nanoparticles was analyzed by spectral data obtained. It showed an absorbance peak around 405nm, specific for silver nanoparticles. Based on the TEM analysis data, the size of silver nanoparticles were approximately around 26.90nm to 34.47nm. The zone of inhibition was measured by using disc diffusion method and the highest zone of inhibition against Bacillus Cereus(18 mm for 25 μl). These nanoparticles were further evaluated for its synergistic effect with ciprofloxacin. Synthesized silver nanoparticles showed the best results against Salmonella Enterica. (47 nm for 20 μl AgNPs+Ciprofloxacin). Conclusions Nepenthes spp. acts as a good source for the biosynthesis of silver nanoparticles. Silver nanoparticles synthesized from the Nepenthes spp. showed good antibacterial activity against Escherichia Coli, Salmonella Enterica, Bacillus Cereus and Staphylococcus Aureus and showed excellent synergistic effect with ciprofloxacin. Hence, because of this strong antibacterial possessed by silver nanoparticles, it may be helpful to counter the bacterial resistance. Moreover, by conducting this study, it will be helpful for researchers as a future reference on the subject of nanotechnology.