Abstract
ID30A-3 (or MASSIF-3) is a mini-focus (beam size 18 µm × 14 µm) highly intense (2.0 × 1013 photons s-1), fixed-energy (12.81 keV) beamline for macromolecular crystallography (MX) experiments at the European Synchrotron Radiation Facility (ESRF). MASSIF-3 is one of two fixed-energy beamlines sited on the first branch of the canted undulator setup on the ESRF ID30 port and is equipped with a MD2 micro-diffractometer, a Flex HCD sample changer, and an Eiger X 4M fast hybrid photon-counting detector. MASSIF-3 is recommended for collecting diffraction data from single small crystals (≤15 µm in one dimension) or for experiments using serial methods. The end-station has been in full user operation since December 2014, and here its current characteristics and capabilities are described.
Highlights
In 2008, the European Synchrotron Radiation Facility (ESRF) launched Phase I of its upgrade program, an integral part of which was the replacement and modernization, as part of the ‘Upgrade Program Beamline 10’ (UPBL10; Theveneau et al, 2013) project, of the ageing ID14 complex with a new suite of end-stations for structural biology
MASSIF-3 is currently recommended for diffraction data collection from small crystals (< 15 mm) or from larger crystals where use of a relatively small beam will allow the use of helical data collections (Flot et al, 2010) or the identification of the best diffracting segment of the crystal for a diffraction experiment
Opened to external users in December 2014, diffraction data collected on MASSIF-3 have, at the time of writing, contributed to 150 publications and have resulted in more than 150 depositions in the Protein Data Bank (PDB; wwPDB consortium, 2019; http://biosync.sbkb. org/stats.do?stats_sec=RGNL&stats_focus_lvl=SITE&stats_site=ESRF)
Summary
In 2008, the ESRF launched Phase I of its upgrade program, an integral part of which was the replacement and modernization, as part of the ‘Upgrade Program Beamline 10’ (UPBL10; Theveneau et al, 2013) project, of the ageing ID14 complex with a new suite of end-stations for structural biology. The MASSIF-specific OH2 houses most optical elements for the MASSIF beamlines as well as a long, lead-shielded vacuum tube which allows the white beam of the ID30B branch pass through the hutch. The transmitted white beam impinges on a liquid-nitrogen-cooled single Si(111) crystal with a bandwidth of 1.5 Â 10À4 used as a monochromator for MASSIF-3, which delivers a beam at a photon energy of 12.812 keV into its experimental hutch (EH2). This energy on the high-energy remote side of the selenium edge was chosen in order to be able to use the anomalous scattering contribution from selenium for de novo phasing experiments. A Basler GigE camera allows to visualize the beam footprint on the YAG, which serves as a scattering element for a diode (read by a Keithley picoammeter) to measure relative beam intensities
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