Id-1: Regulator of EGFR and VEGF and potential target for colorectal cancer therapy

Ibrahim Meteoglu,Muhan Erkus,Nezih Meydan

doi:10.1186/1756-9966-27-69

Abstract

BackgroundThe helix-loop-helix transcription factor Id-1 (an inhibitor of differentiation and DNA binding) plays a role in development and progression of many tumours. Id-1 is known to exert its effects on the epidermal growth factor receptor (EGFR) and the vascular endothelial growth factor (VEGF). The aim of this study was to reveal whether there was a relationship between Id-1 and EGFR and VEGF in colorectal carcinoma.MethodsTumour and non-tumour tissue specimens from 46 cases of colorectal carcinoma were exposed to immunohistochemical staining for Id-1, EGFR and VEGF. The relationship between the degree of staining and tumour grade, tumour stage and all tumour markers was investigated.ResultsTumour cells showed positive staining for Id-1 in 43 cases (93.5%), for EGFR in 41 cases (89%) and for VEGF in 42 cases (91%). There was a significant relation between the tumour grade and the degree of staining for Id-1, EGFR and VEGF. The relation between the tumour stage and the degree of staining for Id-1, EGFR and VEGF was also significant. There was a significant relation between Id-1 expression and EGFR and VEGF expressions. Non-tumoural tissue specimens were not stained with Id-1 and EGFR antibodies in any of the cases, but stained with VEGF antibody in 3 cases.ConclusionThis study revealed that Id-1, EGFR and VEGF took part in development and progression of colorectal carcinomas and that Id-1 was associated with regulations of EGFR and VEGF. The results of this study support the idea that not only EGFR and VEGF but also Id-1 could be new targets in cancer treatment.

Highlights

The helix-loop-helix transcription factor Id-1 plays a role in development and progression of many tumours
Relationships between Tumour Grades and Id-1, epidermal growth factor receptor (EGFR) and vascular endothelial growth factor (VEGF) In order to investigate the relation between colorectal tumour grades and Id-1, EGFR and VEGF, the tumours were divided into three groups: grade I (G I) (n = 10), grade II (G II) (n = 21) and grade III (G III) (n = 15) tumours
Like EGFR, VEGF is a new therapeutic target [7]. It is still debatable whether angiogenesis affects advanced colorectal carcinomas, we found that increased angiogenesis, shown by VEGF, contributed to tumour progression and that Id-1 was associated with VEGF in colorectal carcinomas

Summary

Introduction

The helix-loop-helix transcription factor Id-1 (an inhibitor of differentiation and DNA binding) plays a role in development and progression of many tumours. The aim of this study was to reveal whether there was a relationship between Id-1 and EGFR and VEGF in colorectal carcinoma. Colorectal carcinomas are frequently encountered tumours in the world, especially in developed countries. These tumours account for about 10% of deaths from cancer [1]. They were the second and the third most frequent causes of deaths from cancer in males and females respectively. The conventional treatment for colorectal tumours is surgery. It is known that tumour grade, penetration depth, angiolymphatic invasion and lymph node involvement are important conventional parameters used to determine the prognosis in colorectal tumours [2].

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