ID: 225: New multiplex assay panels for accurate quantification of non-human primates biomarkers

Abstract

Due to the immunological and physiological similarities between non-human primates (NHPs) and humans, understanding the disease progression and immune response in NHP species can predict response in humans. Therefore, NHPs are preferred model systems in drug safety test, vaccine development, and infectious disease and brain research. Correspondingly, accurate measurement of soluble biomarkers in NHPs is important for drug development and biomedical research. We have developed several NHP multi-analyte flow assay panels, including T Helper Cytokine Panel, Inflammation Panel, Growth Factor Panel, Adipokine Panel, and Cytokine/Chemokine Panel, for the quantification of a variety of NHP biomarkers including cytokines (IL-1 β , IL-2, 4, 5, 6, 10, IL-12p40, 17A, IL-17F, IL-21, IL-22, IL-23, IL-33, IFN- γ , TNF- α ) , chemokines (RANTES, MCP-1, MIP-1 α , MIP-1 β , IP-10, IL-8, MIG and I-TAC), growth factors (EGF, Ang-2, G-CSF, GM-CSF, PDGF-AA, PDGF-BB, FGF-b, etc.) and adipokines (Adiponectin, Adiosin, Resistin, Leptin, etc.). These assays are bead-based sandwich immunoassays, with fluorescence-encoded beads that can be separated by a variety of flow cytometers. In each panel, up to thirteen NHP biomarkers can be quantified simultaneously. Each panel has been validated for use in cell culture supernatant, serum, plasma and urine samples for both rhesus and cynomolgus monkey species. These panels provide higher sensitivities and broader dynamic ranges than traditional ELISA methods, offering useful tools for researchers dedicated to NHP research and drug discovery.