Abstract
Previous cases reported recurrent all oocytes in repeated cycles were arrested at MI failed to mature after culture and ICSI resulted in total nonfertilization. Ionomycin induced first PB extrusion in patients with recurrent maturation-resistant MI oocytes. ICSI combined with assisted oocyte activation (AOA) on MII oocytes matured in vitro resulted in abnormal fertilization with no further embryo cleavage potential. The objective of this work was to research if AOA helpful to fertilization for recurrent maturation-resistant MI oocytes. In this case, patient both cycles retrievaled immature MI oocytes characterized by absence perivitelline space (APVS). In the second cycle, ionomycin was used for AOA after MI oocyte perfomed ICSI, no fertilization again.
Highlights
The oocytes classfied germinal vesicle (GV), metaphase I (MI) and metaphase II (MII) after controlled ovarian stimulation (COS) in in vitro fertilization (IVF)
These MI arrest oocytes accompanied with immature fibrous appearance of the zona pellucida proceed further with intracytoplasmic sperm injection (ICSI) resulted in total nonfertilization [5] [8]
We present a case all oocytes at immature MI stage characterized by absence perivitelline space (APVS) with ICSI and assisted oocyte activation (AOA)
Summary
The oocytes classfied germinal vesicle (GV), metaphase I (MI) and metaphase II (MII) after controlled ovarian stimulation (COS) in in vitro fertilization (IVF). MI oocytes has capability to reach MII stage following in-vitro maturation (IVM) or fertilization, and blastocyst development performed with intracytoplasmic sperm injection (ICSI) immediately after denudation [1] [2] [3]. The cases previously reporting recurrent retrieval of all immature MI oocytes that were resistant to IVM [4] [5] [6] [7]. These MI arrest oocytes accompanied with immature fibrous appearance of the zona pellucida proceed further with ICSI resulted in total nonfertilization [5] [8]. We present a case all oocytes at immature MI stage characterized by absence perivitelline space (APVS) with ICSI and AOA
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