ICP-Mass-Spectrometry Ionic Profile of Whole Saliva in Patients with Untreated and Treated Periodontitis.

Federica Romano,Mery Malandrino,Francesca Spadotto,Giovanni Nicolao Berta,Mario Aimetti,Federica Di Scipio,Alexandra Castiblanco

doi:10.3390/biomedicines8090354

Federica Romano, Mery Malandrino + Show 5 more

Open Access

https://doi.org/10.3390/biomedicines8090354

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Journal: Biomedicines	Publication Date: Sep 15, 2020
Citations: 20	License type: CC BY 4.0

Affiliation: University of Turin

Abstract

Over the past decade, there has been growing interest in the association between macro and trace minerals in body fluids and systemic diseases related to chronic inflammation and oxidative stress. Due to the paucity of data in the literature on periodontitis, the aim of this cross-sectional study was to assess the relationship between mineral elements in saliva and periodontal status in patients with untreated and treated periodontitis compared to periodontally healthy controls. Salivary samples from 66 nonsmoker healthy patients (20 periodontally healthy, 24 untreated severe periodontitis and 22 treated severe periodontitis) were analyzed by using inductively coupled plasma mass-spectrometry (ICP-MS). Significant increases in copper (Cu), sodium (Na), iron (Fe) and manganese (Mn) concentrations occurred in saliva of severe periodontitis subjects compared to periodontally healthy controls. No differences were detected between healthy controls and treated periodontitis patients apart from levels of zinc (Zn) and lithium (Li) that were found to be increased and reduced, respectively, in periodontitis group. Most subjects were correctly separated by cluster analysis into active periodontitis and periodontally healthy individuals. Treated periodontitis individuals were classified as healthy subjects. Based on these preliminary results, the assessment of salivary concentration of mineral elements might be useful in discriminating periodontal health and disease.

Highlights

Periodontitis is a widespread chronic inflammatory disease affecting tooth-supporting tissues, initiated and propagated through a complex interaction between bacterial biofilm and host defense system [1]
There were no statistically significant differences when comparing healthy controls with untreated periodontitis patients (p = 0.058) and both periodontitis groups (p = 0.824), while a statistically significant difference emerged between healthy controls and untreated periodontal patients (p = 0.007)
The assessment of salivary concentration of mineral elements might be useful in discriminating periodontal health and disease

Summary

Introduction

Periodontitis is a widespread chronic inflammatory disease affecting tooth-supporting tissues, initiated and propagated through a complex interaction between bacterial biofilm and host defense system [1]. The shift from a symbiotic microflora to a dysbiotic pathogenic community triggers the host inflammatory response that contributes to the periodontal breakdown [2]. Gingival inflammation, soft tissue destruction and alveolar bone loss occur sequentially. Inflammatory mediators, tissue destructive molecules, and metabolites are released into the oral fluids [3]. Qualitative and quantitative changes in the composition of oral fluids could have diagnostic and therapeutic significance [4]. Saliva has the capability to mirror both oral and systemic health conditions since it contains a highly complex mixture of substances originating from multiple local and systemic sources [5].

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Discussion

Conclusion