Abstract
BackgroundNonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat sports-related muscle injuries. However, NSAIDs were recently shown to impede the muscle healing process after acute injury. Migration of skeletal muscle cells is a crucial step during the muscle healing process. The present study was performed to investigate the effect and molecular mechanisms of action of ibuprofen, a commonly used NSAID, on the migration of skeletal muscle cells.MethodsSkeletal muscle cells isolated from the gastrocnemius muscle of Sprague-Dawley rats were treated with ibuprofen. MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was used to evaluate cell viability, and cell apoptosis was evaluated by TUNEL assay, after ibuprofen treatment. Skeletal muscle cell migration and spreading were evaluated using the transwell filter migration assay and F-actin staining, respectively. The protein expression of p130cas and CrkII, which are cell migration facilitating genes, was determined by western blot analysis. The overexpression of p130cas of muscle cells was achieved by p130cas vector transfection.ResultsThe results demonstrated that ibuprofen did not have a significant negative effect on cell viability and apoptosis. Ibuprofen inhibited the migration and spreading of skeletal muscle cells in a dose-dependent manner. Ibuprofen also dose-dependently decreased the protein expression of p130cas and CrkII. Furthermore, overexpression of p130cas resulted in the promotion of cell migration and spreading and counteracted ibuprofen-mediated inhibition.ConclusionThis study suggested that ibuprofen exerts a potentially adverse effect on the migration of skeletal muscle cells by downregulating protein expression of p130cas and CrkII. These results indicate a possible mechanism underlying the possible negative effect of NSAIDs on muscle regeneration.
Highlights
Muscle injuries are one of the most common injuries in sports, and the incidence ranges from 10 to 55% of all injuries [1, 2]
Ibuprofen had no impact on the number of viable skeletal muscle cells In order to investigate cell proliferation, skeletal muscle cells were treated with different concentrations of ibuprofen for 24 h
MTT assay illustrated no significant difference between different concentrations of ibuprofen-treated skeletal muscle cells
Summary
Muscle injuries are one of the most common injuries in sports, and the incidence ranges from 10 to 55% of all injuries [1, 2]. 37% of male professional football players miss training or competition due to muscle (2019) 9:23 healing process after acute injury. A recent study has shown that prostaglandin E2 (PGE2) promoted proliferation of muscle stem cells and NSAIDs that inhibit PGE2 synthesis, may impair muscle regeneration, and lead to weakened muscles [11]. NSAIDs might have a potentially harmful effect on the muscle healing process after acute injury. Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used to treat sports-related muscle injuries. NSAIDs were recently shown to impede the muscle healing process after acute injury. Migration of skeletal muscle cells is a crucial step during the muscle healing process. The present study was performed to investigate the effect and molecular mechanisms of action of ibuprofen, a commonly used NSAID, on the migration of skeletal muscle cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
