Abstract
Glucosinolates (GSLs) extracted from various parts of Iberis sempervirens L., including seeds, stems, leaves, and flowers, were qualitatively and quantitatively assessed. The analyses of GSLs were performed by their desulfo counterparts using the UHPLC-DAD-MS/MS technique and by their volatile breakdown products, isothiocyanates, using the GC-MS technique. The GSL profile comprised various types, including those derived from: methionine, represented by methylsulfinylalkyl GSL (glucoiberin), and methylsulfanylalkyl GSL (glucoibervirin and glucoerucin); phenylalanine (glucotropaeolin); and tryptophan (4-methoxyglucobrassicin). Among these, the highest level of glucoiberin was detected in the leaves, reaching 35.37 µmol/g of dry weight (DW), while the highest level of glucoibervirin was detected in the seeds, reaching 18.51 µmol/g DW. To obtain GSL breakdown products, a variety of isolation methods were employed, including hydrodistillation in a Clevenger-type apparatus (HD), CH2Cl2 after myrosinase hydrolysis for 24 h (EXT), microwave-assisted distillation (MAD), and microwave hydrodiffusion and gravity (MHG). Volatile isolates were tested for their antiproliferative activity using an MTT assay against the human lung cancer cell line A549 and the human bladder cancer cell line T24 during an incubation period of 72 h. HD and MAD showed the best activity against T24, with IC50 values of 0.61 µg/mL and 0.62 µg/mL, respectively, while EXT was the most effective against the A549 cell line, with an IC50 of 1.46 µg/mL.
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