酸漿屬種間雜交之研究（I）不同成熟度雜交種胚培養

Abstract

An unilateral incompatibility mechanism prevents Physalis angulata from serving as the male parent in crosses with Physalis ixocarpa. The media used is the same as that for embryo culture in Lycopersicon sp. Twenty-three days after pollination, embryo callus was produced, up to 7.4%, in the immature embryos of the interspecific cross between Physalis angulata and P. ixocarpa (Purple), and 3.0% between Physalis angulata and P. ixocarpa (Toma Verde). Twenty-seven days after pollination, 1.4% of embryo callus was produced in the immature embryos of the interspecific cross between Physalis angulata and P. ixocarpa (Purple), and 1.1% between Physalis angulata and P. ixocarpa (Toma Verde). Colchicines was used for the treatment of Physalis angulata. When plants regenerated from the chromosome were tetraploid (double-chromosome), 1.0% of embryo callus was produced in the immature embryos of the interspecific cross between Physalis angulata (D) and P. ixocarpa (Purple), and 0.9% between Physalis angulata (D) and P. ixocarpa (Toma Verde). Although percentage of the immature embryos plated for callus was low, this method for production of F1 interspecific hybrids between Physalis angulata and P. ixocarpa is efficient. This paper shows that seeds from the 23-days-old fruits were found with the highest percentage of callus formation.