I -V Simulation of Amperometric Biosensor in Detection of Cancer through Cyclic Voltammetry Technique

Abstract

This electronic A biosensor, which is electro chemical in nature comprises amperometric, potentiometric and conductometric types, and is very versatile in detection of different pathogens. They are also highly useful in detection of different cancers at an early stage. A biosensor is one which has a ligand and a transducer. Ligand refers to a biological element and transducer is the one which converts one form of energy to another. Amperometric biosensors yield current in micro amperes as output. Basing upon the range of current, the state of art can be defined. In this paper the cancer is detected at an early stage using an amperometric biosensor. Cancer is not a pathogen or a microbial cell, but it is unregulated, uncontrolled growth of living cells due to genetic imbalance. The early detection results in early health recovery. The amperometric biosensor can be fabricated with a composition of ferro ferry and glucose oxidase. Ferro ferry which is a start-up potential for amperometric biosensor for detection of pathogens. Electro chemical polymerization of aniline (PANI) test yields amine (NH2) and amene (NH) helps in detection of cancer with high sensitivity, limit of quantification and detection of oxygen content in the blood analyte of interest. The detecting element is glucose oxidase (GOx) which acts as a catalyst and is a ligand in detection of cancer at early and later stage. The detection is based on Michaelis and Menton equation yielding gluconic lactone and oxygen at anode and cathode respectively. The Oxygen content decides the cancer cell activity by measuring its sensitivity to redox potentials. The redox potentials are carried out by an electro chemical method called direct current cyclic voltammetry (DCCV) technique. In this research article, the electrochemical model in MATLAB Simulink was developed and simulated for current versus time and potential versus time. It was found that the current and potential were decreased drastically with respect to input parameters supplied. Cancer can be detected using Amperometric biosensor in DCCV technique in less time and is reliable which makes this method better than existing methods of cancer detection like biopsy, biomarkers.