Abstract
The Langmuir-Blodgett (LB) film technique has been successfully applied to the construction of stable and photo-active films of chromatophore membranes and isolated reaction centers from two species of photosynthetic bacteria, Rhodobacter sphaeroides and Rhodopseudomonas viridis. LB films of these preparations were characterized at the air/water interface through compression isotherms and film stabilities. Films deposited on glass slides were analyzed by spectrophotometric and redox potentiometric techniques. The results obtained indicate that the in vivo properties of the photosynthetic apparatus in the deposited films are essentially unchanged. Furthermore, the pigments and redox cofactors in the films are highly oriented and offer a unique opportunity for structural and functional studies of the kind described in the accompanying paper (Biochim. Biophys. Acta 1057 (1991) 258-272).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
