I know you: familiarity with an audience influences male-male interactions in Siamese fighting fish, Betta splendens

Abstract

The presence of an audience alters male–male interactions in a number of social species. Additionally, whether individuals have interacted previously may influence the current encounter. Although it is clear that previous exposure influences communication and that the presence of an audience affects behavior in a number of contexts, how these phenomena might interact to impact interactions is underexplored. This study examined the effects of audience familiarity on male–male interactions in Siamese fighting fish (Betta splendens). Treatments that varied based on familiarity with and sex of the audience as well as length of time after initial audience exposure were used to investigate whether male aggressive interactions were affected by these factors. Males first encountered an individual that would later become an audience. Pairs of males then interacted with each other in either the presence of this familiar individual or a new, unfamiliar audience conspecific or with no audience at all. Opponent-directed gill flaring and tail beats were influenced by familiarity with the audience individual, although they were affected differently by the sex of the audience present. Gill flaring was affected predominantly by the presence of a male audience, whereas tail beat rate was influenced by the presence of a female audience. Gill flaring rate did not differ regardless of whether 4 or 24 h had passed between the initial exposure to the audience and the testing phase. In contrast, the familiarity effect disappeared after 4 h for tail beats. These results stress the importance of considering the familiarity of individuals within a network when examining communication networks.