I)建立新型水揚酸羥化酶專一隱藏式螢光分子探針生化檢測平台 (II)合成新化合物苯並咪唑衍生物電解液之添加劑提升敏化染料太陽能電池的光電轉換效率η (

Abstract

In this study two, establishing new biosensor and dye-sensitized solar cell (DSSC) are the main projects of this thesis. The first part is the characterzation and application of new latent flourophore. Salicylate hydoxylase (SHL) is flavoenzyme catalyzes a stoichiometric conversion of salicylic acid (SA) to catechol in the presence of NADH and oxygen. .Our laboratory prepared a new latent flourogenic probe, Salicylate hydoxylase Latent fluorophore (SF) for real time monitor the SHL activities in the presence of NADH aerobe condition. The fluorogenic chemical transformation of SF triggered by SHL is through a tandem reaction, decarboxylation, hydroxylation, and quinion-methide-rearrangment reaction. In this study, we fully characterzed the fluorescence revealed of this new latent flourophore, SF, by flourescences spectrofluorometer and HPLC. Further more, the fluorescence response of SF does not influences by the presences of redox agents. Finally, we demonstrated that SF, is applicalle as a part of biosensor for analytes determination in the SHL-coupled dehydrogenases assay by including NAD+. This novel assay platform demonstrates a good relationship in detecting 3-hydroxybutyrate and cholesterol in 200 to 800 nM range, and is applicable for the construction of fiber-optic biosensors in the future clinical diagnostic. The second part of this thesis is the improving of Dye-sensitized solar cells (DSSCs) efficiency by incorporation novel electrolyte additive. DSSC have been intensively studied over the past decade and regarded as a promising low-cost alternative to the conventional solid-state devices. Recently many research have identified electrolytic additive which can improve the photoelectric convection efficiency of DSSC additives drastically increased the Voc but reduce Jsc. A series of 12 new bis-bezimidazole derivatives had been prepared and evaluated their influences as additives the in electrolyte DSSC. There 12 benzimidazole additives in DSSC electrolyte were by meaning the photo to electric conventional efficiency η (%) of DSSC. We had identified 2 additives which we out performed the current electrolytic additives (4-TBP).