Hypoxic Preconditioning Effects of Bone Marrow-derived Culture Mesenchymal Stem Cells on CD31+ Expression, Vascular Endothelial Growth Factors-a (VEGF-A) and Stromal-derived Sactors-1 Alpha (SDF-1α)

A F Muzakkir,I G R Suryawan,T Yusrizal

doi:10.1088/1755-1315/441/1/012161

Abstract

Increasing the ability of survival stem cells can be determined by assessing the state of hypoxic preconditioning (HPC) in stimulated cells. The assessment is carried out through bone marrow-derived stem (BMSCs) cultures obtained from the femoral bone marrow aspiration procedure (Wismar Rat). This study aimed to prove the hypothesis that there is CD31+ expression on HPC cultures; also, the concentration of VEGF-A and SDF-1α were higher in HPC than control. This research is designed as a laboratory experiment for a period of three months. Male Wistar rat (n = 16) was divided into 2 groups: P0 controls (21% [O2]) and P1 treatments (1% [O2]). Each sample was run into eight repetitions and observed for 48 hours. The stages in this study include isolation and culture of BMSCs, identification of phenotypic BMSCs based on the expression of cell-surface markers (CD105+, CD34−) and hypoxic exposure. After that, it was observed that the potential of angiogenesis in samples was based on CD31+ expression, the paracrine activity of vascular endothelial growth factor-A (VEGF-A) and stromal-derived factor-1 Alpha (SDF-1α). The results showed that there is no CD31+ expression on P1. A of VEGF-A and SDF-1⌑ were higher in P1 than P0.

Highlights

Until now, the management of myocardial infarction has been still limited to revascularization and medical therapy, which aims to restore coronary blood flow and reduce the burden on the heart muscle
This study aimed to prove the hypothesis that there is CD31+ expression on hypoxic preconditioning (HPC) cultures; the concentration of vascular endothelial growth factor-A (VEGF-A) and stromal-derived factor-1 α (SDF-1α) were higher in HPC than control
It was observed that the potential of angiogenesis in samples was based on CD31+ expression, the paracrine activity of vascular endothelial growth factor-A (VEGF-A) and stromal-derived factor-1 Alpha (SDF-1α)

Summary

Introduction

The management of myocardial infarction has been still limited to revascularization and medical therapy, which aims to restore coronary blood flow and reduce the burden on the heart muscle. Some of the latest research in the tissue engineering field provides new hope in the treatment of myocardial infarction, taking advantage of pluripotent ability from stem cells, which is transplanted to repair and regenerate heart muscle tissue [1]. Stem cells are immature network precursor cells that can renew themselves and differentiate into various types of cells [2]. Mesenchymal stem cells (MSCs), known as multipotent mesenchymal stromal cells, are cells that can renew themselves and present in several tissues and organs. MSCs have attracted much attention because these cells are isolated from bone marrow aspirates in minimal amounts and can be developed for clinical purposes both in vitro and in vivo [3]

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