Abstract
Aim:To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro.Materials and Methods:Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05).Results:The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05).Conclusion:Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.
Highlights
Mesenchymal stem cells (MSCs) constitute a population of adult stem cells that can transdifferentiate into types of mesodermal lineage cells and into multilineage cell types [1]
Hypoxic preconditioning with 1% O2 increase CXCR4 and stromal derived-cell factor 1 (SDF1) expression
Preliminary studies have shown that MSCs are capable of producing a range of cytokines and growth factors such as basic fibroblast growth factor and vascular endothelial growth factor (VEGF)
Summary
Mesenchymal stem cells (MSCs) constitute a population of adult stem cells that can transdifferentiate into types of mesodermal lineage cells and into multilineage cell types [1]. Preliminary studies have shown that MSCs are capable of producing a range of cytokines and growth factors such as basic fibroblast growth factor and vascular endothelial growth factor (VEGF). The other factors that can mediate such migration are stromal derived-cell factor 1 (SDF1) and C-X-C chemokine receptor type 4 (CXCR4) [2]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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