Abstract
Pretreatment of animals with sublethal doses of endotoxin renders them tolerant to subsequent endotoxin challenge. Tissue hypoxia following infection may potentiate the immune response to injury. To evaluate the effect of hypoxia/reoxygenation (H/R) on endotoxin tolerance by macrophages, adult CBA mice were pretreated with 10 μg/ animal of LPS injected intraperitonealy. Five days later, animals were subjected to 16 hr of hypoxia followed by 2 hr of reoxygenation. Harvested peritoneal macrophages were cultured and either stimulated in vitro with LPS or left unstimulated. Culture supernatants collected at 2, 4, and 6 hr were assayed for tumor necrosis factor (TNF), prostaglandin E2 (PGE 2), and nitric oxide (NO). Control animals underwent the same procedures, but were maintained in normoxia. H/R resulted in significantly greater TNF production but significantly lower PGE 2 and NO production, P < 0.05 by ANOVA. These data suggest that LPS-induced tolerance of macrophages to the same stimulus is abated by H/R.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
