Hypoxia inducible factor-1α, cyclooxygenase-2 and C reactive protein combined examination in the diagnosis of ulcerative colitis

Abstract

Objective To investigate hypoxia-inducible factor-1α （ HIF-1α）, cyclooxygenase-2 （COX-2） and C-reactive protein （CRP） mRNA transcription level of ulcerative colitis pathological tissues and expression levels of HIF-1α, COX-2 and CRP of ulcerative colitis serum. Methods Ninety-eight patients with ulcerative colitis were divided into group A （ mild）, group B （moderate） and group C （severe） according to the severity, with 25 patients of volunteers as the control group D. RT-PCR was used to analyze mRNA levels of HIF-1α, COX-2 and C-reactive protein in ulcerative colitis pathological tissues. ELISA was used to detect the HIF-1α, COX-2 and C-reactive protein levels of serum. Results HIF-1α mRNA levels gradually increased with the exacerbations of ulcerative colitis. COX-2 mRNA levels of group A were signifi- cantly higher than those of Group D （ P 〈 0. 05 ）, COX-2 mRNA levels of Group B were significantly higher than of group A （P 〈 0. 05 ）, and COX-2 mRNA levels of group C have no significant difference compared with group B （ P 〉 0. 05 ）. CRP mRNA levels of group A have no significant difference compared with group D （ P 〉 0. 05 ）, CRP mRNA levels of group B were significantly higher than those of group A （ P 〈 0. 05 ）, and CRP mRNA of group C were significantly higher than group B （ P 〈 0.05 ）. The average serum protein levels of HIF-1α, COX-2 and C-reactive protein gradually increased with the exacerbations of ulcerative coli- tis. Conclusion HIF-1α, COX-2 and C-reactive protein mRNA levels of lesion cells and their serum levels were correlated with the progression of ulcerative colitis positively, suggesting that the detection of HIF-1α,COX-2 and C-reactive protein mRNA levels and expression levels can be used to predict the severity of ulcerative colitis. Key words: Colitis; Hypoxia-inducible factor 1, alpha subunit; Cyclooxygenase 2; C-reactive protein