Hypoxia and warming take sides with small marine protists: An integrated laboratory and field study

Abstract

Hypoxia and ocean warming are two mounting global environmental threats influencing marine ecosystems. However, the interactive effects of rising temperature and depleted dissolved oxygen (DO) on marine protists remains unknown. Here, we conducted a series of laboratory experiments on four protozoa with distinct cell sizes to investigate the combined effects of temperature (19, 22, 25, 28, and 31 °C) and oxygen availability (hypoxia, 2 mg DO L−1 and normoxia, 7 mg DO L−1) on their physiological performances (i.e., growth, ingestion, and respiration rates). The hypoxia-induced inhibition in three physiological rates increased with the biovolume of the protists. As the larger surface area to volume (SA/V) quotients of smaller protists facilitate higher capabilities of oxygen absorption and utilization, the smaller protists suffered less inhibitions induced by hypoxia. Moreover, the hypoxia-induced inhibition in physiological rates was exacerbated by increasing temperature, which can be verified by the reductions in the temperature sensitivities (represented by the activation energy, E). These results suggest that hypoxia could lead to a shift of protistan community with enhanced domination of small protists, and warming could exacerbate such a trend. We further examined our laboratory results in the Pearl River Estuary, where extensive bottom hypoxia often occurs in summer. We found the mean protist biovolume in hypoxic waters was significantly lower than that at normal stations. Also, the mean protist biovolume decreased with declining DO concentration and rising temperature, indicating the interactive effect of temperature and oxygen availability. Collectively, we suggest that hypoxia could cause a higher proportion of small-sized cells in the marine protistan community, and the projected ocean warming could intensify the tendency, which could undermine the capacity of oceanic carbon sequestration.