Hypoxia and lost gills: Respiratory ecology of a temperate larval damselfly

Abstract

Damselfly larvae, important predators and prey in many freshwater communities, may be particularly sensitive to hypoxia because their caudal lamellae (external gills) are frequently lost. In this study, we address how lost lamellae interact with low oxygen to affect respiration and behavior of the widespread North American damselfly Ischnura posita. Results showed no effect of lost lamellae on resting metabolic rate or critical oxygen tension. Ventilation behaviors increased only when dissolved oxygen (DO) was at or below 25% saturation and these behaviors were not affected by the number of lamellae. Use of the oxygen-rich surface layer occurred almost exclusively at the lowest dissolved oxygen level tested (10% saturation, 2.0kPa). Damselflies that were missing lamellae spent more time at the surface than individuals with intact lamellae. The negative relationship between body size and time at the surface, and the negative relationship between body mass and critical oxygen tension suggest that larger I. posita may be more hypoxia tolerant than smaller individuals. Overall, I. posita was minimally affected by missing lamellae and seems well-adapted to low DO habitats. Average critical oxygen tension was very low (0.48kPa, 2.4% saturation), suggesting that individuals can maintain their metabolic rate across a broad range of DO, and behaviors changed only at DO levels below the hypoxia tolerance thresholds of many other aquatic organisms.