Hypoxemia as the mechanism of acute cationic polymer toxicity in rainbow trout and prevention of toxicity using an anionic neutralizing polymer.

Abstract

Industrial operations such as surface mining, road building, and aggregate washing result in high concentrations of suspended particles (Total Suspended Solids; TSS) in surface waters which must be treated prior to discharge into fish-bearing waters. A common industrial practice is to add flocculants to improve the efficacy and speed of TSS sedimentation. A significant environmental issue even small amounts of uncomplexed cationic polymer coagulant/flocculant remaining in treated water is highly toxic to fish at very low concentrations (LC50 ∼ 0.3 mg L-1). Fingerling trout (Oncorhynchus mykiss) were exposed to (1) a cationic flocculant (Water Lynx 800 (WL800), (2) a Clearflow neutralizing polymer (CN369), and (3) a combination of WL800 and CN369 at various ratios with measured LC50 as an index of toxicity. Acute toxicity was entirely reversed by addition of the neutralizing polymer at WL800:CN369 ratios >1:1.5mg/L. Furthermore, we demonstrate that the proximal mechanism of acute cationic polymer toxicity is hypoxemia due to accumulation of polymer on the gill epithelia rather than gill damage. Exposure of 0.5mg/L WL800 reduced oxygen consumption by >50% reduction by 12h and this was accompanied by significantly increased blood, brain, and liver [lactate] and [glucose]. The development of an inexpensive amelioration technique preventing cationic polymer toxicity is a significant advancement in surface and industrial water treatment to prevent cationic polymer mediated fish kills.