Abstract
BackgroundHypothermic treatment is known to protect organs against cardiac arrest (CA) and improves survival rate. However, few studies have evaluated the effects of hypothermia on CA-induced liver damages. This study was designed to analyzed the possible protective effects of hypothermia on the liver after asphyxial CA (ACA). Rats were randomly subjected to 5 min of ACA followed by return of spontaneous circulation (ROSC). Body temperature was controlled at 37 ± 0.5 °C (normothermia group) or 33 ± 0.5 °C (hypothermia group) for 4 h after ROSC. Liver tissues were extracted and examined at 6 h, 12 h, 1 day, and 2 days after ROSC.ResultsThe expression of infiltrated neutrophil marker CD11b and matrix metallopeptidase-9 (MMP9) was investigated via immunohistochemistry. Morphological damage was assessed via hematoxylin and eosin (H & E) staining. Hypothermic treatment improved the survival rate at 6 h, 12 h, 1 day, and 2 days after ACA. Based on immunohistochemical analysis, the expression of CD11b and MMP9 was significantly increased from 6 h after ACA in the normothermia group. However, the expressions of CD11b and MMP9 was significantly decreased in the hypothermia group compared with that of the normothermia group. In addition, in the results of H & E, sinusoidal dilatation and vacuolization were apparent after ACA; however, these ACA-induced structural changes were reduced by the 4 h-long hypothermia.ConclusionsIn conclusion, hypothermic treatment for 4 h inhibited the increases in CD11b and MMP9 expression and reduced the morphological damages in the liver following ACA in rats. This study suggests that hypothermic treatment after ACA reduces liver damages by regulating the expression of CD11b and MMP9.
Highlights
Hypothermic treatment is known to protect organs against cardiac arrest (CA) and improves survival rate
Our study demonstrated that neutrophil infiltration (CD11b immunoreactivity) was significantly increased from 6 h and peaked at 1 day after asphyxial CA (ACA), while hypothermic treatment reduced the ACA-induced increase in the expression of CD11b at each time points, suggesting that hypothermia has a protective effect by inhibiting CD11b expression as well as by reducing the degree of neutrophil infiltration
This study investigated the effect of hypothermia on CD11b/neutrophil and matrix metallopeptidase-9 (MMP9) expressions in the liver I-R injury after ACA
Summary
Hypothermic treatment is known to protect organs against cardiac arrest (CA) and improves survival rate. Few studies have evaluated the effects of hypothermia on CA-induced liver damages. This study was designed to analyzed the possible protective effects of hypothermia on the liver after asphyxial CA (ACA). Body temperature was controlled at 37 ± 0.5 °C (normothermia group) or 33 ± 0.5 °C (hypothermia group) for 4 h after ROSC. Liver ischemiareperfusion (I-R) injury occurs in various clinical conditions such as liver hemorrhage, shock, surgical resection, liver transplantation and cardiac arrest (CA) [2, 3]. The liver I-R injury results in high rate of morbidity and mortality [1, 4]. Liver I-R injury is associated with leukocyte adhesion/
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