Abstract
Many metabolites produced by various microorganisms have proven their usefulness in the area concerning human health. However, most of their diverse natural compound biosyntheses are hardly discovered. These metabolites might have specific or novel functions and these diverse active compounds can be achieved by biosynthesis, semi-biosynthesis, or chemical synthesis. A strategy to exploit the biosynthesis potential of a fungal strain is to use various culture conditions and to evaluate the chemical profiles of the culture extracts. The value of this approach was demonstrated with the fungal strain Aigialus parvus BCC 5311, producer of hypothemycin, aigialospirol, and aigialomycin A-D. The optimization of hypothemycin production and its derivative diversity by Aigialus parvus BCC 5311 was carried out using qualitative (general factorial design) and quantitative analysis (two-level fractional factorial design). Qualitative analysis revealed that soluble starch and yeast extract were shown to be the best carbon and nitrogen source respectively for the production of hypothemycin, aigialospirol and aigialomycin A-D. Quantitative analysis showed that the initial pH of culture medium is the most important factor that affects the production of hypothemycin and its derivatives (aigialospirol and aigialomycin A-D) production. Optimal medium composition used in a 5 L bioreactor generated a specific growth rate of A. parvus BCC 5311 of 0.0295 h-1, biomass yield of 1.6 g×gstarch-1, hypothemycin yield of 13.6 mg×gbiomass-1, and hypothemycin production rate of 0.6 mg×L-1×day-1. The maximum concentration of 58.0 mg×L-1 of hypothemycin was obtained at 120 h of culturing. Furthermore, the Aigialomycin A-D and Aigialospirol obtained were diversified towards various cultural conditions used. The high amount of hypothemycin produced and the diversity of derivatives obtained from this study should be useful for future mass production.
Highlights
Diversifying bioactive metabolites for specific or novel functions and for their usefulness in the area of human health are proved to be attractive by means of biosynthesis, semi-biosynthesis, or chemical synthesis [1,2]
The present study described the identification of the optimal carbon and nitrogen sources influencing hypothemycin and derivatives of Aigiaromycin A-D, Aigialosprirol, and Aigialone production in A. parvus BCC 5311 using a general factorial design and a two-level fractional factorial design
A. parvus BCC 5311 was cultivated on different carbon and nitrogen sources
Summary
Diversifying bioactive metabolites for specific or novel functions and for their usefulness in the area of human health are proved to be attractive by means of biosynthesis, semi-biosynthesis, or chemical synthesis [1,2]. The isolated microorganisms from natural resources might have lost their ability to produce diverse compounds in synthetic medium [3,4], especially for those fungi isolated from insects. This problem concerning the loss of compounds may cause their absence in novel compound discovery or mislead to other compounds of higher bioactivity. Hypothemycin and aigialomycin D exhibit in vitro antimalarial activity with IC50 value of OPEN ACCESS
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
