Abstract
Immunoreactive luteinizing hormone-releasing hormone (LH-RH) was quantitated in hypothalamic extracts of the frog ( Xenopus laevis) and demonstrated to be indistinguishable from synthetic mammalian LH-RH. Hypothalamic immunoreactive LH-RH content varied in relation to season and reproductive physiological state in the frog. Hypothalamic LH-RH content was low in sexually quiescent frogs collected in the nonbreeding season (winter), while LH-RH concentration was high in reproductively active frogs collected in the breeding season (spring). It seems likely, therefore, that environmental cues stimulate the reproductive system in the frog by increasing LH-RH production and secretion.
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