Abstract

Hypothalamic grafts from embryos, larvae, and adults, of the Pacific Treefrog, Hyla regilla, and the Tiger Salamander, Ambystoma tigrinum, homoplastically transplanted to young larvae previously hypophysectomized at the tailbud stage, evoked melanin dispersion and aggregation of reflecting pigment in host chromatophores. To avoid contamination with adenohypophysial hormones hypothalamic tissue was excised from embryos prior to contact with the adenohypophysial anlage and from “albino” larvae and adults hypophysectomized at least one week previously. Sustained responses by adult brain tissue were limited to grafts from ventral parts of the post optic ventral hypothalamus. Responses by grafts from other areas of the hypothalamus and adjacent regions of the brain disappeared within a few days. Histological examination of positive and negative grafts confirmed their exclusively neural origin but did not reveal the cells responsible for the chromatophorotropic activity.

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