Hypothalamic and Hypophyseal Receptors for Estradiol in High and Low Sexually Performing Rams

Abstract

Rams, characterized over a 3-year period as being high ( n = 8) or low ( n = 10) sexual performers, were euthanized to determine if differences in sexual behavior were associated with differences in hypophyseal and hypothalamic receptors for estradiol. Rams were exposed to estrous ewes and courtship behavior was recorded for 5 hr prior to tissue collection. Blood samples for determination of serum concentrations of luteinizing hormone (LH) were collected every 15 min during the behavior testing period. Hourly blood samples were analyzed for serum concentrations of estradiol (E) and testosterone (T). Rams characterized as high (HP) or low (LP) sexual performers had similar LH profiles; there were no differences noted in basal concentration, number of pulses, or pulse amplitude of LH. There were no differences in serum concentrations of T or E during the behavior testing period between HP and LP rams. Unoccupied receptors for E (moles E bound x 10 -15) in the stalk-median eminence (SME) ( P < 0.05) in HP rams (15.1 ± 3.1) were greater than those in LP rams (4.6 ± 1.9). Occupied receptors for E (moles E bound x 10 -15) in the preoptic area (POA) ( P < 0.1) in HP rams (6.0 ± 0.8) tended to be greater than those in the LP rams (3.8 ± 0.8). The proportion of total E receptors (occupied + unoccupied) in the POA that were occupied was greater ( P < 0.05; 0.41 ± 0.03 vs 0.23 ± 0.04) for HP than for LP rams. In contrast, HP rams had less ( P < 0.05) occupied E receptors (moles E bound x 10 -15) in the anterior pituitary gland (AP) than LP rams (152.0 ± 38.5 vs 337.3 ± 51.7). The proportion (%) of E receptors that were occupied in the AP in HP rams also tended to be less ( P < 0.06) than that in LP rams (8 ± 2 vs 14 ± 1). No differences were detected in occupied or unoccupied E receptors in the medial basal hypothalamus, medial amygdala, or stalk-median eminence. In conclusion, differences in occupied receptors for E in the POA and the AP, and unoccupied receptors for E in the SME, were associated with differences in ram sexual behavior.