Hypolipidemic and Hepatoprotective Effects of Polysaccharides Extracted from Liriope spicata Var. Prolifera in C57BL/6J Mice with High-Fat Diet-Induced Hyperlipidemia

Zhi-Nan Xiang,Chen Chen,Luo-Sheng Wan,Fadia S Youssef,Yi-Hui Liu,Jia-Chun Chen

doi:10.1155/2020/8013189

Abstract

In this study, C57BL/6J mice with high-fat diet- (HFD-) induced hyperlipidemia were treated with total Liriope spicata var. prolifera polysaccharides (TLSP: 200, 400, and 800 mg/kg body weight), simvastatin (3 mg/kg body weight), or saline for 8 weeks, respectively. The results showed that TLSP had strong lipid-lowering and hepatoprotective effects on C57BL/6J mice with HFD-induced hyperlipidemia. TLSP administration significantly reduced serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels and downregulated the expressions of peroxisome proliferator-activated receptor (PPAR)γ and fatty acid synthase (FAS) in the adipose and liver tissues of the mice. TLSP exerted hypolipidemic and hepatoprotective effects by activating lipid/bile acid metabolism via the FXH-SHP/CYP7A1 and SEBP-1c/FAC/ACC signaling pathways. Thus, TLPS is a promising natural polymer with hepatoprotective and hypolipidemic properties.

Highlights

Hyperlipidemia is characterized by an elevation in the levels of total cholesterol (TC), triglycerides (TGs), and lowdensity lipoprotein cholesterol (LDL-C) along with a decline in the level of high-density lipoprotein cholesterol (HDL-C)
Erefore, in the present study, we evaluated the hypolipidemic effects of TLSP in C57BL/6J mice with highfat diet- (HFD-) induced hyperlipidemia, and the mRNA expressions of genes related to cholesterol, bile acid, and fatty acid metabolism are measured to explore the mechanisms underlying the effects of TLSP on lipid metabolism
TLSP treatment decreased the high-fat diet- (HFD-)induced gain in body weight in a dose- and time-dependent manner but did not significantly affect the food intake of C57BL/6J mice, as compared with the High-Fat Diet (HFD) group. ese data suggested that TLSP reduced the food efficiency ratio (FER) induced by the HFD

Summary

Introduction

Hyperlipidemia is characterized by an elevation in the levels of total cholesterol (TC), triglycerides (TGs), and lowdensity lipoprotein cholesterol (LDL-C) along with a decline in the level of high-density lipoprotein cholesterol (HDL-C). Various pharmaceutical agents have been applied in the treatment of hyperlipidemia, including statins, ezetimibe, and fibrates Such interventions have shown promising effectiveness in lipid management but can cause many side effects like liver injury, muscle aches, and digestive dysfunction. We have previously demonstrated that the total polysaccharide extract isolated from this tuberous root is composed of two fructans, named LSP1 and LSP2 (Figure 1), which have an average molecular weight of 3.20 and 4.29 kDa, respectively. Both fructans have the same backbone structure consisting of β-(1-2)-fructosyl residues. Erefore, in the present study, we evaluated the hypolipidemic effects of TLSP in C57BL/6J mice with highfat diet- (HFD-) induced hyperlipidemia, and the mRNA expressions of genes related to cholesterol, bile acid, and fatty acid metabolism are measured to explore the mechanisms underlying the effects of TLSP on lipid metabolism

Materials and Methods

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Results

Conclusion