Hypoglycemic Effect of Antioxidant Astaxanthin from Vaname Shrimp Waste Extract Using Soybean Oil in Streptozotocin-Induced Mice (Mus Musculus)

Abstract

Vaname shrimp (Litopenaeus vannamei) waste contains powerful biological compound called astaxanthin which has high antioxidant activity compared to other carotenoids pigment or α-tokopherol. In this study, the vaname shrimp waste were extracted using soybean oil and was used to treat streptozotocin-induced diabetic mice. The astaxanthin of vaname shrimp waste extract was counted using spectrophotemer and the antioxidant activity was determined by using DPPH method. The astaxanthin concentration was found highest in pigmented oil phase with the amount of 209.616 ppm and the IC50 value of the pigmented oil phase was 122.744 ppm. Hypoglycemic effect was evaluated in streptozotocin-induced mice which were devided into 5 groups and received different oral treatment in each group (glibenclamide in dose 5 mg/bw, aquadest 0.2 ml and astaxanthin in dose of 10, 15 and 20 mg/bw). The blood glucose levels were examined every 2 days for 14 days. The treatment of astaxanthin from vaname shrimp waste with dose 20 mg/bw showed remarkable fall on blood glucose in streptozotosin-induced diabetic mice, while a slight fall can be seen in dose 10 and 15 mg/kg bw. Therefore, these result present that vaname shrimp waste extract contained high astaxanthin and had high antioxidant activity which can be effectively used to reduce blood glucose in streptozotocin-induced diabetic mice.