Hypoglycemic and anti-lipidemic Evaluation of Fruit Extract and Solvent Fractions of Ziziphus oenoplia in Streptozotocin-Induced Diabetic Rats

Abstract

Stem Bark, Root and fruit of Ziziphus oenoplia (Rhamnaceae) has been used in traditional health systems to treat diabetes and many more disorders in southern Asia as India, Sri Lanka and Burma. However hypoglycemic and hypo-lipidemic potential of fruit of this valuable plant is not scientifically validated till date. The aim of present study is to evaluate hypoglycemic and hypo-lipidemic effect of ethanol extracts and solvent fractions of Ziziphus oenoplia fruit. The ethanol extracts and solvent fractions in different dose concentration 100, 200, 400 mg/kg of Ziziphus oenoplia fruit were evaluated for hypo-glycemic effect in single dose and multiple dose study for 21 days as well as for hypo-lipidemic potential in streptozotocin induced diabetic rats. The outcome of present study indicates, crude extract and solvent fractions significantly reduce blood glucose level on normoglycemic and diabetic rats.The acute oral toxicity study of Z. oenoplia fruit extract did not show mortality in the animals at the limit dose of 2g/kg. In STZ induced diabetic rat, maximum BGL lowering effect produced by EEZO400 followed by CF400 after 21 days of treatment. Total cholesterol, triglycerides and low-density lipoprotein was also significantly decreased. The observed result may be due to active principles present in extract and fractions. The result showed the potential effects of Z.oenoplia fruit extract and fractions as hypoglycemic and anti hyper-lipidemic in dose dependant manner compare to glibenclemide as standard drug. The claimed traditional use as anti-diabetic has scientific back ground.