HYPOGLYCAEMIC ACTIVITY OF CENTRATHERUM ANTHELMINTICU IN EXPERIMENTAL ANIMALS

Abstract

The Effect of Ethanolic extracts of Centratherum anthelminticum on Diabetic rats Assessment of the antihyperglycemic effect of ethanolic extract of Centratherum anthelminticum seeds in normal and alloxan induced diabetic rats: The animals will be divided into nine groups and each group consisted of six rats. Group 1 as normal control. Group 2 as the diabetic control. Groups 3, 4 and 5 are normally treated with 0.25 g, 0.50 g and 0.75 g/kg BW of CAEt, respectively. Groups 6, 7 and 8 are diabetic rats treated with 0.25 g, 0.50 g, and 0.75 g/kg BW of CAEt, respectively. Group 9 are diabetic rats which received glibenclamide (0.02 g/kg BW) an oral hypoglycemic agent dissolved in distilled water. Normal control and the diabetic control rats are fed distilled water alone. After an overnight fast, the plant extract suspended in distilled water will feed to the rats by gastric intubation using a force feeding needle. Blood samples are collected for the measurement of blood glucose from the tail vein at 0, 1, 2, 3, 4, 5 and 6 h after the administration of plant extract and blood glucose levels are determined by Glucose oxidase – peroxidase method. The phytochemical analysis will be carried out in the ethanolic extract by different methods of phytochemical analysis. Keywords: Hypoglycaemia, Diabetes mellitus, Centratherum anthelminticu m The effect of Ethanolic extracts of Centratherum anthelminticum on Diabetic rats Assessment of antihyperglycemic effect of ethanolic extract of Centratherum anthelminticum seeds in normal and alloxan induced diabetic rats: The animals will be divided in to nine groups and each group consisted of six rats. Group 1 as normal control. Group 2 as the diabetic control. Groups 3, 4 and 5 are normal treated with 0.25 g, 0.50 g and 0.75 g/kg bw of CAEt, respectively. Groups 6, 7 and 8 are diabetic rats treated with 0.25 g, 0.50 g and 0.75 g/kg bw of CAEt, respectively. Group 9 are diabetic rats which received glibenclamide (0.02 g/kg bw) an oral hypoglycemic agent dissolved in distilled water. Normal control and the diabetic control rats are fed distilled water alone. After an overnight fast, the plant extract suspended in distilled water will fed to the rats by gastric intubation using a force feeding needle. Blood samples are collected for the measurement of blood glucose from the tail vein at 0, 1, 2, 3, 4, 5 and 6 h after the administration of plant extract and blood glucose levels are determined by Glucose oxidase – peroxidase method.Phytochemical analysis will carried out in the ethanolic extract by different methods of phytochemical analysis. Keywords: Hypoglycaemia, Diabetes mellitus, Centratherum anthelminticum