Hypocholesterolemic Effect of Avocado (Persea americana Mill) Juice in Hypercolestrolemic Rats Model

Abstract

BackgroundHypercholesterolemia is one of the risk factors of cardiovascular disease. Avocado (Persea americana Mill) is a popular fruit in tropical countries and has several benefits for health. This study aims to determine the hypocholesterolemic effect of avocado juice on the hypercholesterolemic rats model. Method: The subjects were male Wistar rats (Rattus novergicus) aged 2-3 months. The rats were divided into 5 groups consisting of negative control (NC), positive control (C), Treatment 1 (P1) with a low-dose of avocado juice (2 ml), Treatment 2 (P2) with moderate-dose (3 ml), and Treatment 3 (P3) with high-dose (4 ml). Groups C, P1, P2, and P3 have induced hypercholesterolemia with 4 ml quail egg yolk for four weeks. Then groups P1, P2, and P3 were given avocado juice for four weeks. All rats were taken plasma samples after hypercholesterolemia induction and intervention with avocado juice to check for total cholesterol levels. All data were statistically analyzed with The One-Way ANOVA test and Bonferroni Post-Hoc test. Values were considered significant at p<0.05. Result: Mean of total cholesterol (nmol/L) after hypercholesterolemia induction in both groups (NC group, C group, P1 group, P2 group, and P3 group) is 2.50±0.01, 5.98±0,09, 5.70±0.08, 5.89±0.05, and 6.06±0.09. After intervention with avocado juice, total cholesterol in both groups is 2.48±0.01, 5.75±0.04, 3.97±0.03, 3.41±0.05, and 2.85±0.06. The One-Way ANOVA test and Bonferroni Post-Hoc test showed significant change (p<0.001). Conclusion: Avocado juice can decrease total cholesterol levels in the hypercholesterolemic rats model.