Abstract
Axillary buds from 1-year-old twigs of European aspen (Populus tremula L.) were used for propagation of indigenous aspen clones for further phytoremedial studies. The most intense bud development was seen after the break of dormancy, i.e. from the middle of February until mid-March. Lower infection rates were observed from the axillary buds collected at an urban forest border in comparison to a forest location, accompanied by higher percentages of browning. The optimal medium for shoot induction primarily via callus cultures was Murashige and Skoog mineral salts medium (MS) supplemented by 20 μM zeatin riboside and 1 μM indol acetic acid. The shoots were rooted in half-strength MS medium supplemented by the same hormones, after which they were successfully transferred to a commercial soil mixture. The protocol for aspen propagation is proposed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
