Hyphantria cunea Nucleopolyhedrovirus, a Novel Baculovirus Isolated from Fall Webworm in Korea

Abstract

∗Department of Plant Medicine, College of Agriculture Life & Environment Science, Chungbuk National University, Cheongju361-763, Korea(Received 11 April 2011; Accepted 18 May 2011)To elucidate the novelty of Hyphantria cunea nucle-opolyhedrovirus (HcNPV) isolated in Korea, polyhe-drin and inhibitor of apoptosis (iap) gene structureswere determined and analyzed. The analysis ofHcNPV polyhedrin showed a little difference with97.6% at the nucleotide level but no difference at theamino acid level when compared with that of previ-ously reported H. cunea NPV (HycuNPV). On theother hand, iap genes showed variable differences with89.0-99.6% nucleotide and 90.0-99.6% amino acidsequence identities. Especially, the 5’ and 3’ non-cod-ing flanking sequences of iap1 gene had lower degreeof identity with those of HycuNPV. Although the phy-logenetic analyses using polyhedrin and iap genesshowed that HcNPV is closely related with HycuNPV,we could provide that HcNPV is a novel isolate havingnovel gene structures. Key words: Hyphantria cunea nucleopolyhedrovirus,HcNPV, Polyhedrin, iap