Hyphal transport of 15 N-labelled nitrogen by a vesicular-arbuscular mycorrhizal fungus and its effect on depletion of inorganic soil N.

Abstract

Hyphal transport of nitrogen from a 15 N-labelled ammonium source by a VA-mycorrhizal fungus was studied under controlled experimental conditions. Cucumis sativus L. cv. Aminex (F1 hybrid) was grown alone or together with Glomus intraradices Schenck and Smith in containers with a hyphal compartment separated from the rooting medium by a fine nylon mesh. Lateral movement of the applied 15 N towards the roots was minimized by using a nitrification inhibitor (N-serve) and a hyphal buffer compartment. Recovery of 15 N by mycorrhizal and non-mycorrhizal plants was 6 and 0%, respectively, after a labelling period of 23 days. The corresponding figures, without N-serve added, were 4 and 7%. A prolongation of the labelling period by 8 days (N-serve applied) resulted in an increase in the 15 N recovery by mycorrhizal plants to 30% of the applied 15 N. Non-mycorrhizal plants contained only traces of 15 N. The external hyphae depleted the soil in the hyphal compartment efficiently for inorganic N. In contrast, hyphal compartments of control containers still contained considerable amounts of inorganic N. The 15 N assimilated by the external hyphae in one hyphal compartment was not translocated in significant amounts to the external hyphae in another hyphal compartment. The possible implication of this for inter-plant N transfer by VA hyphal connections is discussed.