Abstract

A material's refractive index is a major input for the light-scattering analysis of microscopic particles and label-free optical imaging of biological tissues. Existing techniques for measuring the refractive index of a nonspherical specimen over a wide wavelength range are typically slow, because they require recording so many orientations and wavelengths of the laser illuminating the specimen. Here researchers demonstrate hyperspectral three-dimensional (3D) refractive-index imaging at high throughput using snapshot optical tomography, which can record a 3D map of the refractive index in a single snapshot, in combination with a wavelength-scanning laser.

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