Abstract
Abstract. The development of improved preservation techniques and the reliable assessment of donor grafts are main fields of research in transplantation medicine. Normothermic machine perfusion (NMP) is a promising alternative to static cold storage of organs, maintaining physiological conditions during preservation. In combination with NMP, we introduce hyperspectral imaging (HSI) as a novel approach for the monitoring of physiological kidney parameters. A line-scan HSI camera system was used to record images of porcine kidneys during NMP. Based on a dual-wavelength algorithm, the oxygen saturation levels were calculated from HSI recordings. Furthermore, we observed HSI images in the near-infrared (NIR) range in order to detect water characteristics of the kidney tissue. We found increasing levels of oxygenation during NMP and could discriminate between perfused and non-perfused areas. Cysts at the renal capsula were characterized by an absorption increase in the NIR band. Within this work, we showed that HSI is able to detect relevant chemical changes during NMP and allows the identification of pathologic variations.
Highlights
The current situation in transplantation medicine is characterized by an acute lack of available donor grafts, in the field of kidney transplantation
In this work we presented a novel approach for the monitoring and visualization of relevant chemical changes of isolated kidneys prior to transplantation
We conclude that camerabased measurements such as hyperspectral imaging (HSI) are easy to integrate in a perfusion setup and allow a fast and noninvasive measurement of tissue characteristics
Summary
The current situation in transplantation medicine is characterized by an acute lack of available donor grafts, in the field of kidney transplantation. In order to expand the donor pool, an increasing number of marginal organs from donors with pre-existing risk factors (e.g., donors with hypertension or diabetes mellitus, age > 70 years or death from cerebrovascular accident) are considered for transplantation (Reese et al, 2006). These organs are often associated with functional limitations (Querard et al, 2016; Pascual et al, 2008). In the range of 700–1300 nm, called bio-optical window, the light penetration in tissue has its maximum depth (Jobsis, 1977).
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