Abstract
Hyperspectral imaging technique was employed to determine spatial distributions of chlorophyll (Chl), and carotenoid (Car) contents in cucumber leaves in response to angular leaf spot (ALS). Altogether, 196 hyperspectral images of cucumber leaves with five infection severities of ALS were captured by a hyperspectral imaging system in the range of 380–1,030 nm covering 512 wavebands. Mean spectrum were extracted from regions of interest (ROIs) in the hyperspectral images. Partial least square regression (PLSR) models were used to develop quantitative analysis between the spectra and the pigment contents measured by biochemical analyses. In addition, regression coefficients (RCs) in PLSR models were employed to select important wavelengths (IWs) for modelling. It was found that the PLSR models developed by the IWs provided the optimal measurement results with correlation coefficient (R) of prediction of 0.871 and 0.876 for Chl and Car contents, respectively. Finally, Chl and Car distributions in cucumber leaves with the ALS infection were mapped by applying the optimal models pixel-wise to the hyperspectral images. The results proved the feasibility of hyperspectral imaging for visualizing the pigment distributions in cucumber leaves in response to ALS.
Highlights
Ultraviolet spectrophotometry and high performance liquid chromatography have been used to determine Chl and Car contents through a series of operations including weighting, grinding, extracting supernatant, and measuring[8,9]
The specific objectives were to: (1) extract important wavelengths (IWs) from hyperspectral data for establishing the optimal quantitative relationships between spectra and pigment contents; (2) establish regression models for predicting Chl and Car contents and evaluate the performance of these quantitative analyses; (3) visualize Chl and Car concentration distributions in cucumber leaves in response to angular leaf spot (ALS) based on the selected hyperspectral images
Statistics of the pigments measured by biochemical analyses
Summary
Ultraviolet spectrophotometry and high performance liquid chromatography have been used to determine Chl and Car contents through a series of operations including weighting, grinding, extracting supernatant, and measuring[8,9]. These methods involve labor-intensive, time-consuming and tedious extraction procedures. Hyperspectral imaging technique combines reflectance spectra with image processing It has promoted recent progress in remote sensing and has been widely used for mapping the distribution of physiological characteristics in plants[17]. The specific objectives were to: (1) extract important wavelengths (IWs) from hyperspectral data for establishing the optimal quantitative relationships between spectra and pigment contents; (2) establish regression models for predicting Chl and Car contents and evaluate the performance of these quantitative analyses; (3) visualize Chl and Car concentration distributions in cucumber leaves in response to ALS based on the selected hyperspectral images
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