Abstract
The paper presents a microscope equipped with a hyperspectral ocular nozzle with a phase diffraction grating, which is used as a dispersing element. The spectral range of the device is 400–1000 nm, with the spectral resolution of 5 nm. The paper describes a microscope software that is used to solve problems of spectral analysis.
Highlights
Hyperspectral equipment that can distinguish a large number of spectral channels, in contrast to conventional three-color imaging, when using hyperspectral image processing, allows you to see subtle color differences in the visible range of optical radiation, as well as wavelengths invisible to the human eye in the nearinfrared range [1]
In addition to the classical search signatures, in biology and medicine, a number of compounds with the use of illumination of certain wavelengths can cause the effect of photoluminescence, fluorescence, Raman scattering, etc., which allows you to use the hyperspectral equipment in criminology [3], materials science [4], mineralogy [5]
Using a microscope-spectrometer, it is possible to obtain the spectrum of individual pixels in the wavelength range from 400 nm to 1000 nm with a spectral resolution of 5 nm [10,11], depending on the dispersing element used and the resolution of the recording matrix
Summary
Hyperspectral equipment that can distinguish a large number of spectral channels, in contrast to conventional three-color imaging, when using hyperspectral image processing, allows you to see subtle color differences in the visible range of optical radiation, as well as wavelengths invisible to the human eye in the nearinfrared range [1]. This ability makes it possible to distinguish a number of chemical compounds of the underlying surface in remote sensing of the Earth, and is increasingly used in biology and medicine [2]. Using a microscope-spectrometer, it is possible to obtain the spectrum of individual pixels in the wavelength range from 400 nm to 1000 nm with a spectral resolution of 5 nm [10,11], depending on the dispersing element used and the resolution of the recording matrix
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