Hypersalinity and hydrogen peroxide upregulation of gene expression of antioxidant enzymes in Ulva fasciata against oxidative stress.

Abstract

The modulation of manganese superoxide dismutase (MnSOD), FeSOD, ascorbate peroxidase (APX), glutathione reductase (GR), and catalase (CAT) gene expression and activities and antioxidants in Ulva fasciata against hypersalinity (90 per thousand)-induced oxidative stress was studied. Increases in H(2)O(2) contents but no changes in lipid peroxidation and protein carbonyl group contents suggest oxidative damage did not occur in 90 per thousand condition. Antioxidants were consumed for reactive oxygen species (ROS) scavenging indicated by decreased ascorbate and glutathione contents by 90 per thousand. Antioxidant enzymes were differently expressed by 90 per thousand for ROS removal. MnSOD activity and transcript increased 1 h after 90 per thousand treatment with a peak at hour 3, while FeSOD activity increased fast to the plateau after 1 h and its transcript increased after 3 h. APX activity increased 1 h after 90 per thousand but its transcript rose till 3 h, and GR activity increased after 1 h with a peak at hour 3 but its transcript increased till 3 h. CAT activity and transcript increased after 12 h. Enzyme activity is transcriptionally regulated by 90 per thousand except a fast increase in FeSOD, APX, and GR activities during 1 h. APX is responsible for early H(2)O(2) decomposition while CAT scavenges H(2)O(2) in the later period. The inhibition of 90 per thousand induced increase of H(2)O(2) content and FeSOD activity and transcript by treatment of a H(2)O(2) scavenger, dimethylthiourea, and the increase of FeSOD transcript of 30 per thousand grown thalli by H(2)O(2) treatment suggest that H(2)O(2) mediates the upregulation of FeSOD by hypersalinity while other enzymes is modulated by factors other than H(2)O(2).